Anticorps Polyclonal de lapin anti-YIPF4

YIPF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 15473-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 15473-1-PBS

  HeLa cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15473-1-AP (same clone as 15473-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 15473-1-PBS

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 15473-1-AP (same clone as 15473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 15473-1-AP (same clone as 15473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15473-1-AP (same clone as 15473-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using YIPF4 antibody (15473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15473-1-AP (same clone as 15473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15473-1-AP (YIPF4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL594-phalloidin staining of F-actin. This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15473-1-PBS cible YIPF4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YIPF4 Protéine recombinante Ag7777
• Nom complet: Yip1 domain family, member 4
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC004875
• Symbole du gène: YIPF4
• Identification du gène (NCBI): 84272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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