Anticorps Monoclonal anti-YME1L1

• Phare
• Validé par KD/KO

YME1L1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B7E4

N° de cat : 66551-1-PBS

Synonymes

2B7E4, ATP-dependent metalloprotease FtsH1, ATP-dependent zinc metalloprotease YME1L1, EC:3.4.24.-, FTSH

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Galerie de données de validation

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  WB analysis of HeLa using 66551-1-Ig (same clone as 66551-1-PBS)

  WB result of YME1L1 antibody (66551-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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  WB analysis using 66551-1-Ig (same clone as 66551-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66551-1-Ig (same clone as 66551-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66551-1-PBS cible YME1L1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: YME1L1 Protéine recombinante Ag2069
• Nom complet: YME1-like 1 (S. cerevisiae)
• Masse moléculaire calculée: 716 aa, 80 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC023507
• Symbole du gène: YME1L1
• Identification du gène (NCBI): 10730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

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