Anticorps Monoclonal anti-YTHDF1

• Phare
• Validé par KD/KO

YTHDF1 Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A2H12

N° de cat : 66745-1-PBS

Synonymes

3A2H12, C20orf21, DACA-1, DF1, YTH domain-containing family protein 1

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 66745-1-Ig (same clone as 66745-1-PBS)

  WB result of YTHDF1 antibody (66745-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF1 transfected HepG2 cells. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66745-1-Ig (same clone as 66745-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 66745-1-Ig (same clone as 66745-1-PBS)

  IP result of anti-YTHDF1 (IP:66745-1-Ig, 4ug; Detection:66745-1-Ig 1:2000) with Jurkat cells lysate 2000 ug. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66745-1-PBS cible YTHDF1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: YTHDF1 Protéine recombinante Ag11633
• Nom complet: YTH domain family, member 1
• Masse moléculaire calculée: 559 aa, 61 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC050284
• Symbole du gène: YTHDF1
• Identification du gène (NCBI): 54915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.

{{ptg:RelatedPrimaryAntibodies}}