Anticorps Polyclonal de lapin anti-YWHAZ

• Phare
• Validé par KD/KO

YWHAZ Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14881-1-PBS

Synonymes

14-3-3, 14-3-3 protein zeta/delta, KCIP 1, KCIP-1

---

Galerie de données de validation

• 

  WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14881-1-AP (same clone as 14881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14881-1-AP (same clone as 14881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14881-1-AP (YWHAZ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 14881-1-AP (same clone as 14881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14881-1-AP (YWHAZ antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14881-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14881-1-PBS cible YWHAZ dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YWHAZ Protéine recombinante Ag6654
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC003623
• Symbole du gène: YWHAZ
• Identification du gène (NCBI): 7534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAZ.

{{ptg:RelatedPrimaryAntibodies}}