Anticorps Monoclonal anti-YWHAZ

YWHAZ Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G12B10

N° de cat : 67698-1-PBS

Synonymes

14-3-3, 14-3-3 protein zeta/delta, 3G12B10, KCIP 1, KCIP-1

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Galerie de données de validation

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  WB analysis using 67698-1-Ig (same clone as 67698-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67698-1-Ig (YWHAZ antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67698-1-Ig (same clone as 67698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67698-1-Ig (YWHAZ antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67698-1-Ig (same clone as 67698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67698-1-Ig (YWHAZ antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67698-1-Ig (same clone as 67698-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using YWHAZ antibody (67698-1-Ig, Clone: 3G12B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 67698-1-Ig (same clone as 67698-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.2 μg YWHAZ Monoclonal antibody (67698-1-Ig, Clone:3G12B10) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005), and 0.2 μg Mouse IgM Isotype Control (65243-1-Ig, Clone: MOPC-104E) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67698-1-PBS cible YWHAZ dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: YWHAZ Protéine recombinante Ag6667
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC003623
• Symbole du gène: YWHAZ
• Identification du gène (NCBI): 7534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

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