Anticorps Polyclonal de lapin anti-YY1

• Phare
• Validé par KD/KO

YY1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 22156-1-AP

Synonymes

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of Jurkat using 22156-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 cells using 22156-1-AP

  A549 cells (shcontrol and shRNA of YY1) were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000.

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  WB analysis using 22156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22156-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22156-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22156-1-AP

  IP Result of anti-YY1 (IP:22156-1-AP, 5ug; Detection:22156-1-AP 1:700) with HeLa cells lysate 880ug.

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  IHC staining of human breast cancer using 22156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22156-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 22156-1-AP (YY1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A549, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below
• ChIP: See 10 publications below

Informations sur le produit

22156-1-AP cible YY1 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YY1 Protéine recombinante Ag17792
• Nom complet: YY1 transcription factor
• Masse moléculaire calculée: 414 aa, 45 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC037308
• Symbole du gène: YY1
• Identification du gène (NCBI): 7528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.

Publications

Species	Application	Title
human	IF	Sci Transl Med; Yin Yang 1 protein ameliorates diabetic nephropathy pathology through transcriptional repression of TGFβ1.; Authors - Pan Gao
human	WB	Mol Cell; Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.; Authors - Wenqi Xu
human	WB	Theranostics; KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.; Authors - Chengwan Zhang ; KD Validated
human	ChIP	Cancer Lett; MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.; Authors - Qi Sun
human	WB,IP,ChIP	Free Radic Biol Med; Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation.; Authors - Wentao Wang
mouse	IHC	Cancers (Basel); HPV8 Reverses the Transcriptional Output in Lrig1 Positive Cells to Drive Skin Tumorigenesis.; Authors - Adnan Shahzad Syed