Anticorps Monoclonal anti-YY1

• Phare
• Validé par KD/KO

YY1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, singe, souris et plus (1)

Applications

WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

36

CloneNo.

2E11C5

N° de cat : 66281-1-Ig

Synonymes

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of HeLa using 66281-1-Ig

  Western blot of YY1 in various human cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 66281-1-Ig

  Western blot of YY1 in rat (C6 and ROS1728) and mouse(NIH3T3 and Raw264.7) cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66281-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66281-1-Ig

  WB result of YY1 antibody (66281-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YY1 transfected MCF-7 cells.

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  WB analysis of NIH/3T3 using 66281-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66281-1-Ig

  Western blot of Hela cells with mouse anti-YY1 monoclonal antibody (66281-1-Ig) at various dilutions incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 66281-1-Ig

  COS-7 cells were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 66281-1-Ig

  IP Result of anti-YY1 (IP:66281-1-Ig, 5ug; Detection:66281-1-Ig 1:1000) with NIH/3T3 cells lysate 4000ug.

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  IHC staining of human breast cancer using 66281-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66281-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66281-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66281-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66281-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66281-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66281-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66281-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66281-1-Ig (YY1 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66281-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66281-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:800 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66281-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules A431, cellules COS-7, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'amygdalite humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 27 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 5 publications below
• RIP: See 1 publications below

Informations sur le produit

66281-1-Ig cible YY1 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: YY1 Protéine recombinante Ag17732
• Nom complet: YY1 transcription factor
• Masse moléculaire calculée: 414 aa, 45 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC037308
• Symbole du gène: YY1
• Identification du gène (NCBI): 7528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

Publications

Species	Application	Title
human		Cell; Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription.; Authors - Rui Xiao
mouse	WB	Nat Commun; miR-29b contributes to multiple types of muscle atrophy.; Authors - Jin Li
human	WB,IF,IP	Oncogene; AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression; Authors - Sicheng Wan
mouse	WB	Mol Ther Nucleic Acids; Angiotensin II-induced muscle atrophy via PPARγ suppression is mediated by miR-29b.; Authors - Jin Li
human	WB	Cell Death Dis; CircHIPK3 promotes colorectal cancer growth and metastasis by sponging miR-7.; Authors - Kaixuan Zeng
human	WB	Cancers (Basel); MiR-302b as a Combinatorial Therapeutic Approach to Improve Cisplatin Chemotherapy Efficacy in Human Triple-Negative Breast Cancer.; Authors - Alessandra Cataldo

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