Anticorps Recombinant de lapin anti-YY1

YY1 Recombinant Antibody for WB, IF/ICC, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

1B5

N° de cat : 82712-3-RR

Synonymes

1B5, DELTA, Delta transcription factor, INO 80S, INO80S

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Galerie de données de validation

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  WB analysis using 82712-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82712-3-RR (YY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 82712-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-3-RR, Clone: 1B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 82712-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-3-RR, Clone: 1B5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  ChIP experiment of K-562 using 82712-3-RR

  Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of YY1 (82712-3-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 82712-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82712-3-RR against Human YY1 were performed. The affinity constant is 0.835 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en ChIP-qPCR: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82712-3-RR cible YY1 dans les applications de WB, IF/ICC, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: YY1 Protéine recombinante Ag17792
• Nom complet: YY1 transcription factor
• Masse moléculaire calculée: 414 aa, 45 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC037308
• Symbole du gène: YY1
• Identification du gène (NCBI): 7528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

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