Anticorps Recombinant de lapin anti-YY1

YY1 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2K12

N° de cat : 82712-5-PBS

Synonymes

NF-E1, INO80S, INO 80S, Delta transcription factor, DELTA

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Galerie de données de validation

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  IF Staining of HepG2 using 82712-5-RR (same clone as 82712-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82712-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82712-5-RR (same clone as 82712-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82712-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82712-5-RR (same clone as 82712-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug YY1 Recombinant antibody (82712-5-RR, Clone:2K12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82712-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82712-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82712-5-RR against Human YY1 were performed. The affinity constant is 0.412 pM.

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Informations sur le produit

82712-5-PBS cible YY1 dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: YY1 Protéine recombinante Ag17792
• Nom complet: YY1 transcription factor
• Masse moléculaire calculée: 414 aa, 45 kDa
• Numéro d’acquisition GenBank: BC037308
• Symbole du gène: YY1
• Identification du gène (NCBI): 7528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

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