- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-YY1
YY1 Monoclonal Antibody for IF/ICC
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, rat, singe, souris
Applications
IF/ICC
Conjugaison
CoraLite®488 Fluorescent Dye
CloneNo.
2E11C5
N° de cat : CL488-66281
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF/ICC | cellules HepG2, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL488-66281 cible YY1 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, rat, singe, souris
| Réactivité | Humain, rat, singe, souris |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | YY1 Protéine recombinante Ag17732 |
| Nom complet | YY1 transcription factor |
| Masse moléculaire calculée | 414 aa, 45 kDa |
| Poids moléculaire observé | 65-70 kDa |
| Numéro d’acquisition GenBank | BC037308 |
| Symbole du gène | YY1 |
| Identification du gène (NCBI) | 7528 |
| Conjugaison | CoraLite®488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 491 nm / 516 nm |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL488 YY1 antibody CL488-66281 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
