Anticorps Polyclonal de lapin anti-ZAK

• Phare
• Validé par KD/KO

ZAK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 28761-1-AP

Synonymes

MAP3K20, Leucine zipper- and sterile alpha motif-containing kinase, HCCS-4, HCCS 4, EC:2.7.11.25

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Galerie de données de validation

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  WB analysis using 28761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28761-1-AP (ZAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28761-1-AP

  WB result of ZAK antibody (28761-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZAK transfected HeLa cells.

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  IHC staining of human colon cancer using 28761-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28761-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse tongue using 28761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat tongue using 28761-1-AP

  Immunohistochemical analysis of paraffin-embedded rat tongue tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28761-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZAK antibody (28761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MDA-MB-231
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu lingual de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

28761-1-AP cible ZAK dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZAK Protéine recombinante Ag30599
• Nom complet: sterile alpha motif and leucine zipper containing kinase AZK
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 91kDa, 51 kDa
• Numéro d’acquisition GenBank: BC001401
• Symbole du gène: ZAK
• Identification du gène (NCBI): 51776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.

Publications

Species	Application	Title
human	WB	Genome Biol; The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model; Authors - Yunfei Li ; KD Validated
human	WB	J Cell Mol Med; Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals; Authors - Weiming Ma
human,mouse	WB,IF	Inflammation; ZAKα Induces Pyroptosis of Colonic Epithelium Via the Caspase-11/GSDMD Pathway to Aggravate Colitis; Authors - Song Li

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