Anticorps Monoclonal anti-ZAK

ZAK Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2H10E4

N° de cat : 68599-1-Ig

Synonymes

2H10E4, AZK, EC:2.7.11.25, HCCS 4, HCCS-4

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Galerie de données de validation

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  WB analysis using 68599-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of rat skeletal muscle using 68599-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit skeletal muscle using 68599-1-Ig

  rabbit skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, tissu de muscle squelettique de lapin, tissu de muscle squelettique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68599-1-Ig cible ZAK dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat

• Réactivité: Humain, Lapin, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZAK Protéine recombinante Ag6747
• Nom complet: sterile alpha motif and leucine zipper containing kinase AZK
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 91 kDa
• Numéro d’acquisition GenBank: BC001401
• Symbole du gène: ZAK
• Identification du gène (NCBI): 51776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.

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