Anticorps Monoclonal anti-ZAK

ZAK Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, rat

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H10E4

N° de cat : 68599-1-PBS

Synonymes

2H10E4, AZK, EC:2.7.11.25, HCCS 4, HCCS-4

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Galerie de données de validation

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  WB analysis using 68599-1-Ig (same clone as 68599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 68599-1-Ig (same clone as 68599-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit skeletal muscle using 68599-1-Ig (same clone as 68599-1-PBS)

  rabbit skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68599-1-PBS cible ZAK dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat

• Réactivité: Humain, Lapin, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZAK Protéine recombinante Ag6747
• Nom complet: sterile alpha motif and leucine zipper containing kinase AZK
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 91 kDa
• Numéro d’acquisition GenBank: BC001401
• Symbole du gène: ZAK
• Identification du gène (NCBI): 51776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.

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