Anticorps Recombinant de lapin anti-ZAK

ZAK Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250559E1

N° de cat : 81161-3-PBS

Synonymes

AZK, EC:2.7.11.25, HCCS 4, HCCS-4, Leucine zipper- and sterile alpha motif-containing kinase

---

Galerie de données de validation

• 

  IHC staining of human liver cancer using 81161-3-RR (same clone as 81161-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81161-3-RR (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81161-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 81161-3-RR (same clone as 81161-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81161-3-RR (ZAK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81161-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 81161-3-RR (same clone as 81161-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ZAK antibody (81161-3-RR, Clone: 250559E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81161-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81161-3-RR

  Biolayer interferometry (BLl) kinetic assays of 81161-3-RR against Human ZAK were performed. The affinity constant is 2.57 nM.

---

Informations sur le produit

81161-3-PBS cible ZAK dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZAK Protéine recombinante Ag30599
• Nom complet: sterile alpha motif and leucine zipper containing kinase AZK
• Masse moléculaire calculée: 91 kDa
• Numéro d’acquisition GenBank: BC001401
• Symbole du gène: ZAK
• Identification du gène (NCBI): 51776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38 gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa (ZAK alpha), 51 kDa (ZAK beta) and 35 kDa.

{{ptg:RelatedPrimaryAntibodies}}