Anticorps Monoclonal anti-ZAP70

• Phare
• Validé par KD/KO

ZAP70 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A11G9

N° de cat : 60200-1-PBS

Synonymes

STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein, 70 kDa zeta associated protein

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Galerie de données de validation

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  WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

  WB result of ZAP70 antibody (60200-1-Ig, 1:10000) with si-control and si-ZAP70 transfected Jurkat cells.. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  WB analysis of pig spleen using 60200-1-Ig (same clone as 60200-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  WB analysis of pig thymus using 60200-1-Ig (same clone as 60200-1-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60200-1-Ig (ZAP70 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51134-1

  Cytometric bead array standard curve of MP51134-1, ZAP70 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60200-3-PBS. Detection antibody: 60200-1-PBS. Standard:Ag7943. Range: 0.391-100 ng/mL.

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Informations sur le produit

60200-1-PBS cible ZAP70 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZAP70 Protéine recombinante Ag7943
• Nom complet: zeta-chain (TCR) associated protein kinase 70kDa
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC039039
• Symbole du gène: ZAP70
• Identification du gène (NCBI): 7535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

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