Anticorps Polyclonal de lapin anti-ZBP1

• Phare
• Validé par KD/KO

ZBP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13285-1-PBS

Synonymes

C20orf183, DAI, DLM 1, DLM1, Tumor stroma and activated macrophage protein DLM-1

---

Galerie de données de validation

• 

  WB analysis using 13285-1-AP (same clone as 13285-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13285-1-AP (ZBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13285-1-AP (same clone as 13285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13285-1-AP (same clone as 13285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13285-1-AP (same clone as 13285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13285-1-AP (same clone as 13285-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (13285-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

13285-1-PBS cible ZBP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZBP1 Protéine recombinante Ag4119
• Nom complet: Z-DNA binding protein 1
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 42-68 kDa
• Numéro d’acquisition GenBank: BC028218
• Symbole du gène: ZBP1
• Identification du gène (NCBI): 81030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).

{{ptg:RelatedPrimaryAntibodies}}