Anticorps Recombinant de lapin anti-ZBP1

ZBP1 Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241582E2

N° de cat : 84396-2-PBS

Synonymes

241582E2, C20orf183, DAI, DLM 1, DLM1

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP01267-1

  Sandwich ELISA standard curve of MP01267-1, Human ZBP1 Recombinant Matched Antibody Pair - PBS only. 84396-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4119. 84396-2-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

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  Sample test of MP01267-1

  The mean ZBP1 concentration was determined to be 7.1 ng/mL in HeLa cell extract based on a 2.8 mg/mL extract load, 15.4 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 9.3 ng/mL in HL-60 cell extract based on a 1.4 mg/mL extract load.

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  IHC staining of human colon cancer using 84396-2-RR (same clone as 84396-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84396-2-RR (ZBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84396-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84396-2-RR (same clone as 84396-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (84396-2-RR, Clone: 241582E2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84396-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01267-1

  Cytometric bead array standard curve of MP01267-1, ZBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84396-1-PBS. Detection antibody: 84396-2-PBS. Standard: Ag4119. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84396-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84396-2-RR against Human ZBP1 were performed. The affinity constant is 5.12 nM.

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Informations sur le produit

84396-2-PBS cible ZBP1 dans les applications de IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZBP1 Protéine recombinante Ag4119
• Nom complet: Z-DNA binding protein 1
• Masse moléculaire calculée: 46 kDa
• Numéro d’acquisition GenBank: BC028218
• Symbole du gène: ZBP1
• Identification du gène (NCBI): 81030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).

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