Anticorps Polyclonal de lapin anti-ZBTB20

• Phare
• Validé par KD/KO

ZBTB20 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23987-1-PBS

Synonymes

Dendritic-derived BTB/POZ zinc finger protein, DPZF, Zinc finger and BTB domain-containing protein 20, Zinc finger protein 288, ZNF288

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Galerie de données de validation

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  WB analysis using 23987-1-AP (same clone as 23987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 23987-1-AP (same clone as 23987-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 23987-1-AP (same clone as 23987-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 23987-1-AP (same clone as 23987-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 23987-1-AP (same clone as 23987-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23987-1-AP (same clone as 23987-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 23987-1-AP (same clone as 23987-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 23987-1-AP (same clone as 23987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 23987-1-AP (same clone as 23987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23987-1-AP (same clone as 23987-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 23987-1-AP (same clone as 23987-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23987-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23987-1-PBS cible ZBTB20 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZBTB20 Protéine recombinante Ag21140
• Nom complet: zinc finger and BTB domain containing 20
• Masse moléculaire calculée: 668 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC029041
• Symbole du gène: ZBTB20
• Identification du gène (NCBI): 26137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZBTB20 (zinc finger and BTB domain containing 20), also known as HOF, DPZF, ODA-8S or ZNF288, is a 741 amino acid protein that localizes to the nucleus and contains one BTB (POZ) domain and five C2H2-type zinc fingers. Expressed in thymus, spleen, lymph node and fetal liver, ZBTB20 exists as either a monomer or a homodimer that is thought to function as a transcription factor, playing a role in hematopoiesis, oncogenesis and immune responses. Multiple isoforms of ZBTB20 exist due to alternative splicing events.

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