Anticorps Polyclonal de lapin anti-ZBTB7B

ZBTB7B Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11341-1-AP

Synonymes

c Krox, hcKrox, THPOK, ZBTB15, ZBTB7B, Zfp 67, ZFP67, Zinc finger protein 67 homolog, Zinc finger protein Th POK, ZNF857B

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Galerie de données de validation

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  WB analysis using 11341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11341-1-AP (ZBTB7B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11341-1-AP

  IP result of anti-ZBTB7B(IP:11341-1-AP, 4ug; Detection:11341-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IF Staining of HeLa using 11341-1-AP

  Immunofluorescent analysis of Hela cells, using ZBTB7B antibody 11341-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of MCF-7 using 11341-1-AP

  Immunofluorescent analysis of HepG2 cells, using ZBTB7B antibody 11341-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11341-1-AP cible ZBTB7B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZBTB7B Protéine recombinante Ag1888
• Nom complet: zinc finger and BTB domain containing 7B
• Masse moléculaire calculée: 539 aa, 58 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC012070
• Symbole du gène: ZBTB7B
• Identification du gène (NCBI): 51043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZBTB7B belongs to a large family of transcription factors, generally acting as repressors, characterized by a carboxy-terminal DNA binding domain made of multiple zinc fingers (four in Thpok) and an amino-terminal BTB-POZ domain that mediates homo- (and possibly hetero-) dimerization [PMID: 17084908]. Zbtb7b is up-regulated by MHC-II-restricted thymocytes during their CD4 differentiation and is a major determinant of CD4 lineage choice. Two properties of ZBTB7B deserve attention. First, although Thpok is expressed in a wide variety of cells, its expression in the thymus is highly lineage-specific : CD4 SP thymocytes (and all CD4 T cells) express Thpok, whereas DP and CD8 SP thymocytes do notSecond, both loss- and gain-of-function experiments indicate that Thpok affects lineage choice but not positive selection [PMID: 15729333, 10550051].

Publications

Species	Application	Title
hamster	WB	Acta Biomater; LAP2β transmits force to upregulate genes via chromatin domain stretching but not compression; Authors - Jian Sun
human	WB,IHC	J Transl Med; Epigenetic DNA methylation of Zbtb7b regulates the population of double-positive CD4+CD8+ T cells in ulcerative colitis.; Authors - Hao-Ming Xu
bovine	WB,IP	J Agric Food Chem; Acylated Ghrelin Activates PI3K/mTOR Signaling Pathway by Promoting ThPOK Acetylation to Promote Milk Fat Synthesis in Bovine Mammary Epithelial Cells; Authors - Jiaxin Wang