Anticorps Polyclonal de lapin anti-ZBTB7B

ZBTB7B Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11341-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 11341-1-AP (same clone as 11341-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11341-1-AP (ZBTB7B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11341-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11341-1-AP (same clone as 11341-1-PBS)

  IP result of anti-ZBTB7B (IP:11341-1-AP, 4ug; Detection:11341-1-AP 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 11341-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11341-1-AP (same clone as 11341-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBTB7B antibody (11341-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11341-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11341-1-PBS cible ZBTB7B dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZBTB7B Protéine recombinante Ag1888
• Nom complet: zinc finger and BTB domain containing 7B
• Masse moléculaire calculée: 539 aa, 58 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC012070
• Symbole du gène: ZBTB7B
• Identification du gène (NCBI): 51043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZBTB7B belongs to a large family of transcription factors, generally acting as repressors, characterized by a carboxy-terminal DNA binding domain made of multiple zinc fingers (four in Thpok) and an amino-terminal BTB-POZ domain that mediates homo- (and possibly hetero-) dimerization [PMID: 17084908]. Zbtb7b is up-regulated by MHC-II-restricted thymocytes during their CD4 differentiation and is a major determinant of CD4 lineage choice. Two properties of ZBTB7B deserve attention. First, although Thpok is expressed in a wide variety of cells, its expression in the thymus is highly lineage-specific : CD4 SP thymocytes (and all CD4 T cells) express Thpok, whereas DP and CD8 SP thymocytes do notSecond, both loss- and gain-of-function experiments indicate that Thpok affects lineage choice but not positive selection [PMID: 15729333, 10550051].

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