Anticorps Polyclonal de lapin anti-ZC3H12D

• Phare
• Validé par KD/KO

ZC3H12D Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 24991-1-AP

Synonymes

C6orf95, MCP induced protein 4, MCPIP4, p34, Probable ribonuclease ZC3H12D, TFL, ZC3H12D

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Galerie de données de validation

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  WB analysis of Raji using 24991-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24991-1-AP (ZC3H12D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24991-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24991-1-AP (ZC3H12D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 24991-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24991-1-AP (ZC3H12D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 24991-1-AP

  IP Result of anti-ZC3H12D (IP:24991-1-AP, 4ug; Detection:24991-1-AP 1:600) with Raji cells lysate 2000ug.

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  IHC staining of human tonsillitis using 24991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24991-1-AP (ZC3H12D Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 24991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24991-1-AP (ZC3H12D Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules K-562
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

24991-1-AP cible ZC3H12D dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZC3H12D Protéine recombinante Ag21770
• Nom complet: zinc finger CCCH-type containing 12D
• Masse moléculaire calculée: 527 aa, 58 kDa
• Poids moléculaire observé: 55-58 kDa
• Numéro d’acquisition GenBank: BC157832
• Symbole du gène: ZC3H12D
• Identification du gène (NCBI): 340152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
	WB	Nat Commun; Extracellular mRNA transported to the nucleus exerts translation-independent function.; Authors - Takeshi Tomita ; KO Validated
human	WB	Comput Struct Biotechnol J; Integrating knowledge of protein sequence with protein function for the prediction and validation of new MALT1 substrates; Authors - Peter A Bell
mouse	WB	J Biol Chem; Monocyte Chemotactic Protein-induced Protein 1 and 4 Form a Complex but Act Independently in Regulation of Interleukin-6 mRNA Degradation.; Authors - Shengping Huang ; KD Validated
human	WB	Front Aging Neurosci; DNA Methylation Profiling Reveals the Change of Inflammation-Associated ZC3H12D in Leukoaraiosis.; Authors - Wen-Qing Huang
human	WB	Oncol Lett; miR-128-3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D.; Authors - Maoshu Zhu