Anticorps Recombinant de lapin anti-ZC3H12D

ZC3H12D Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240464C5

N° de cat : 83507-2-RR

Synonymes

MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95

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Galerie de données de validation

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  WB analysis of Raji using 83507-2-RR

  Raji cells were subjected to SDS PAGE followed by western blot with 83507-2-RR (ZC3H12D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83507-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12D Recombinant antibody (83507-2-RR, Clone:240464C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83507-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83507-2-RR against Human ZC3H12D were performed. The affinity constant is 0.343 nM.

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Applications testées

• Résultats positifs en WB: cellules Raji,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83507-2-RR cible ZC3H12D dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZC3H12D Protéine recombinante Ag21770
• Nom complet: zinc finger CCCH-type containing 12D
• Masse moléculaire calculée: 527 aa, 58 kDa
• Poids moléculaire observé: 55-58 kDa
• Numéro d’acquisition GenBank: BC157832
• Symbole du gène: ZC3H12D
• Identification du gène (NCBI): 340152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.

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