Anticorps Polyclonal de lapin anti-ZCCHC8

• Phare
• Validé par KD/KO

ZCCHC8 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 23374-1-AP

Synonymes

ZCCHC8

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Galerie de données de validation

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  WB analysis using 23374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23374-1-AP (ZCCHC8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 23374-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 23374-1-AP (ZCCHC8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 23374-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 23374-1-AP (ZCCHC8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23374-1-AP (ZCCHC8 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 23374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23374-1-AP (ZCCHC8 antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 23374-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23374-1-AP (ZCCHC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, tissu de thymus de souris
• Résultats positifs en IHC: tissu testiculaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

23374-1-AP cible ZCCHC8 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZCCHC8 Protéine recombinante Ag19985
• Nom complet: zinc finger, CCHC domain containing 8
• Masse moléculaire calculée: 707 aa, 79 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC065918
• Symbole du gène: ZCCHC8
• Identification du gène (NCBI): 55596
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. ZCCHC8 acts as a scaffold, mediating the interaction between RBM7 and MTR4. NEXT is involved in exosome-mediated surveillance and decay of noncoding RNAs, such as enhancer RNAs (eRNAs) and aberrant 3′-extended transcripts from small nuclear RNA (snRNA), telomerase RNA, and replication-dependent histone genes. NEXT is an important node in regulating nuclear exosome activities and takes part in several biological processes, including DNA damage response, stress response, and viral ribogenesis . ZCCHC8 also has a robust interaction with YTHDC1 (PMID: 28984244).

Publications

Species	Application	Title
mouse	WB,IP	Mol Cell; Nuclear RNA catabolism controls endogenous retroviruses, gene expression asymmetry, and dedifferentiation; Authors - Denis Torre ; KD Validated
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
human	CoIP	Nucleic Acids Res; Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2.; Authors - Linlin Hou
mouse	WB	Cell Rep; Nuclear Exosome Targeting Complex Core Factor Zcchc8 Regulates the Degradation of LINE1 RNA in Early Embryos and Embryonic Stem Cells.; Authors - You Wu ; KO Validated
human	WB	Nat Neurosci; Globally reduced N6-methyladenosine (m6A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration; Authors - Yini Li ; KD Validated
human	WB	PLoS Genet; RBMX involves in telomere stability maintenance by regulating TERRA expression; Authors - Jingfan Liu