Anticorps Polyclonal de lapin anti-ZDHHC13

ZDHHC13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 24759-1-AP

Synonymes

Huntingtin-interacting protein 14-related protein, HIP3RP, HIP14-related protein, HIP14L, EC:2.3.1.225

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Galerie de données de validation

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  WB analysis of mouse kidney using 24759-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 24759-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24759-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 24759-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24759-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 24759-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 24759-1-AP (ZDHHC13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 24759-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24759-1-AP (ZDHHC13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, cellules Neuro-2a, tissu cutané de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

24759-1-AP cible ZDHHC13 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZDHHC13 Protéine recombinante Ag21607
• Nom complet: zinc finger, DHHC-type containing 13
• Masse moléculaire calculée: 622 aa, 71 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC056152
• Symbole du gène: ZDHHC13
• Identification du gène (NCBI): 54503
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Palmitoyltransferase ZDHHC13 also named as HIP14L or DHHC13 is a 622 amino acid protein, which contains one DHHC-type zinc finger and six ANK repeats. ZDHHC13 localizes in the Golgi apparatus membrane and belongs to the DHHC palmitoyltransferase family. ZDHHC13 is a palmtoyltransferase for HD and GAD2, meanwhile it mediates Mg2+ transportation. ZDHHC13 exists as three alternative splicing isoforms. The molecular weight of isoform 2 is 51 kDa, but we often detected a 45-50kDa protein by western blot.

Publications

Species	Application	Title
human	IF, WB	J Cell Biol; Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.; Authors - Thaddaeus Mutugi Nthiga

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