Anticorps Recombinant de lapin anti-ZDHHC5

ZDHHC5 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242192A4

N° de cat : 84803-4-PBS

Synonymes

242192A4, DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5

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Galerie de données de validation

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  WB analysis using 84803-4-RR (same clone as 84803-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84803-4-RR (ZDHHC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 84803-4-RR (same clone as 84803-4-PBS)

  IP result of anti-ZDHHC5 (IP:84803-4-RR, 4ug; Detection:84803-4-RR 1:1000) with HEK-293 cells lysate 1720 ug. This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84803-4-RR (same clone as 84803-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84803-4-RR (same clone as 84803-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84803-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84803-4-RR against Human ZDHHC5 were performed. The affinity constant is 2.16 nM.

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Informations sur le produit

84803-4-PBS cible ZDHHC5 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZDHHC5 Protéine recombinante Ag15022
• Nom complet: zinc finger, DHHC-type containing 5
• Masse moléculaire calculée: 715 aa, 78 kDa
• Poids moléculaire observé: 75-77 kDa
• Numéro d’acquisition GenBank: BC026967
• Symbole du gène: ZDHHC5
• Identification du gène (NCBI): 25921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

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