Anticorps Recombinant de lapin anti-ZDHHC5

ZDHHC5 Recombinant Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

242192A4

N° de cat : 84803-4-RR

Synonymes

242192A4, DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5

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Galerie de données de validation

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  WB analysis using 84803-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84803-4-RR (ZDHHC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 84803-4-RR

  IP result of anti-ZDHHC5 (IP:84803-4-RR, 4ug; Detection:84803-4-RR 1:1000) with HEK-293 cells lysate 1720 ug.

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  IHC staining of human ovary cancer using 84803-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84803-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84803-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84803-4-RR against Human ZDHHC5 were performed. The affinity constant is 2.16 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84803-4-RR cible ZDHHC5 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZDHHC5 Protéine recombinante Ag15022
• Nom complet: zinc finger, DHHC-type containing 5
• Masse moléculaire calculée: 715 aa, 78 kDa
• Poids moléculaire observé: 75-77 kDa
• Numéro d’acquisition GenBank: BC026967
• Symbole du gène: ZDHHC5
• Identification du gène (NCBI): 25921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

Publications

Species	Application	Title
		J Virol; Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation; Authors - Qisheng Qian

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