Anticorps Monoclonal anti-ZEB1

ZEB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

CloneNo.

1H1F1

N° de cat : 66279-1-Ig

Synonymes

1H1F1, AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8

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Galerie de données de validation

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  WB analysis using 66279-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66279-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 66279-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66279-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66279-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66279-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66279-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66279-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66279-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66279-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 66279-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZEB1 antibody (66279-1-Ig, Clone: 1H1F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules SKOV-3
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IF: See 1 publications below

Informations sur le produit

66279-1-Ig cible ZEB1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZEB1 Protéine recombinante Ag17118
• Nom complet: zinc finger E-box binding homeobox 1
• Masse moléculaire calculée: 1125 aa, 124 kDa
• Poids moléculaire observé: 190-210 kDa
• Numéro d’acquisition GenBank: BC112392
• Symbole du gène: ZEB1
• Identification du gène (NCBI): 6935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa.

Publications

Species	Application	Title
human	WB	Theranostics; FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation; Authors - Xingyan Xu
human	WB	Biochim Biophys Acta Mol Cell Res; The calcium pump PMCA4 prevents epithelial-mesenchymal transition by inhibiting NFATc1-ZEB1 pathway in gastric cancer.; Authors - Teng Wang
human	WB	Breast Cancer; Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells.; Authors - Yingzi Zhang
human	WB	J Clin Med; A Mutation in ZNF143 as a Novel Candidate Gene for Endothelial Corneal Dystrophy.; Authors - Yonggoo Kim
human	WB	Front Oncol; Identification of hub genes within the CCL18 signaling pathway in hepatocellular carcinoma through bioinformatics analysis; Authors - Jinlei Mao
mouse	IF	Am J Physiol Lung Cell Mol Physiol; NFkB/RelA Signaling in Secretoglobin Progenitors Mediates Plasticity and MMP-Induced Barrier Disruption In House Dust Mite-Induced Allergic Asthma; Authors - Melissa E Skibba

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