Anticorps Recombinant de lapin anti-ZEB1

ZEB1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

243139F8

N° de cat : 85729-1-RR

Synonymes

AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8, TCF-8

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Galerie de données de validation

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  WB analysis using 85729-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 85729-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 85729-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85729-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 85729-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85729-1-RR (ZEB1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85729-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85729-1-RR (ZEB1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 85729-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZEB1 antibody (85729-1-RR, Clone: 243139F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of NIH/3T3 using 85729-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ZEB1 antibody (85729-1-RR, Clone: 243139F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85729-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85729-1-RR against Human ZEB1 were performed. The affinity constant is 0.693 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules HepG2, cellules NIH/3T3, tissu de côlon de rat, tissu de côlon de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85729-1-RR cible ZEB1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZEB1 Protéine recombinante Ag16204
• Nom complet: zinc finger E-box binding homeobox 1
• Masse moléculaire calculée: 1125 aa, 124 kDa
• Poids moléculaire observé: 190-210 kDa
• Numéro d’acquisition GenBank: BC112392
• Symbole du gène: ZEB1
• Identification du gène (NCBI): 6935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa.

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