Anticorps Polyclonal de lapin anti-ZEB2

• Phare
• Validé par KD/KO

ZEB2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

57

N° de cat : 14026-1-AP

Synonymes

KIAA0569, SIP 1, SIP1, Smad interacting protein 1, SMADIP1, ZEB2, ZFHX1B, ZFX1B

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Galerie de données de validation

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  WB analysis using 14026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14026-1-AP (ZEB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14026-1-AP (ZEB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14026-1-AP

  WB result of ZEB2 antibody (14026-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZEB2 transfected HepG2 cells.

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  IHC staining of human renal cell carcinoma using 14026-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14026-1-AP (ZEB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14026-1-AP (ZEB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14026-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14026-1-AP (ZEB2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de gliome humain, tissu de cancer du poumon humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 52 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

14026-1-AP cible ZEB2 dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZEB2 Protéine recombinante Ag5171
• Nom complet: zinc finger E-box binding homeobox 2
• Masse moléculaire calculée: 136 kDa
• Poids moléculaire observé: 150-170 kDa
• Numéro d’acquisition GenBank: BC060819
• Symbole du gène: ZEB2
• Identification du gène (NCBI): 9839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )

Publications

Species	Application	Title
mouse	IF	Nat Commun; Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.; Authors - Hans I-Chen Harn
human	WB,IF	Cell Death Differ; Regulation of Semaphorin3A in the process of cutaneous wound healing.; Authors - Yang Zheng
human	WB	Oncotarget; Pygopus-2 promotes invasion and metastasis of hepatic carcinoma cell by decreasing E-cadherin expression.; Authors - Sheng Zhang
human	WB	Cell Death Dis; PIM1 mediates epithelial-mesenchymal transition by targeting Smads and c-Myc in the nucleus and potentiates clear-cell renal-cell carcinoma oncogenesis.; Authors - Bin Zhao
human	WB	J Gastroenterol; LncRNA n339260 functions in hepatocellular carcinoma progression via regulation of miRNA30e-5p/TP53INP1 expression.; Authors - Tieju Liu
human,mouse	WB,IHC	Mol Cancer Ther; The DNA Endonuclease Mus81 Regulates ZEB1 Expression and Serves as a Target of BET4 Inhibitors in Gastric Cancer.; Authors - Yuping Yin