Anticorps Recombinant de lapin anti-ZEB2

ZEB2 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1K22

N° de cat : 82020-1-PBS

Synonymes

SIP1, 1K22, HRIHFB2411, KIAA0569, SIP 1

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Galerie de données de validation

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  WB analysis using 82020-1-RR (same clone as 82020-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82020-1-RR (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 82020-1-RR (same clone as 82020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82020-1-RR (ZEB2 antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 82020-1-RR (same clone as 82020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82020-1-RR (ZEB2 antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 82020-1-RR (same clone as 82020-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ZEB2 antibody (82020-1-RR, Clone: 1K22 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 82020-1-RR (same clone as 82020-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ZEB2 antibody (82020-1-RR, Clone: 1K22 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82020-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82020-1-RR against Human ZEB2 were performed. The affinity constant is 0.682 nM.

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Informations sur le produit

82020-1-PBS cible ZEB2 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZEB2 Protéine recombinante Ag5171
• Nom complet: zinc finger E-box binding homeobox 2
• Masse moléculaire calculée: 136 kDa
• Poids moléculaire observé: 140-150 kD
• Numéro d’acquisition GenBank: BC060819
• Symbole du gène: ZEB2
• Identification du gène (NCBI): 9839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. PMID: 27659015 )

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