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Anticorps Polyclonal de lapin anti-ZFC3H1

ZFC3H1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 30834-1-AP

Synonymes

CCDC131, CSRC2, KIAA0546, PSRC2

Galerie de données de validation

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  • Western Blot (WB) analysis of various lysates using ZFC3H1 Polyclonal antibody (30834-1-AP)

    WB analysis using 30834-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30834-1-AP (ZFC3H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ZFC3H1 Polyclonal antibody (30834-1-AP)

    IF Staining of U2OS using 30834-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZFC3H1 antibody (30834-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Applications testées

Résultats positifs en WBcellules DU 145, cellules HEK-293T
Résultats positifs en IF/ICCcellules U2OS,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

30834-1-AP cible ZFC3H1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ZFC3H1 Protéine recombinante Ag34224
Nom complet zinc finger, C3H1-type containing
Masse moléculaire calculée226 kDa
Poids moléculaire observé250 kDa
Numéro d’acquisition GenBankBC156732
Symbole du gène ZFC3H1
Identification du gène (NCBI) 196441
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZFC3H1, also known as CCDC131 and PSRC2, is a large protein (1989 amino acids), with a C3H1- type zinc finger motif in its center, and contains five tetratricopeptide (TRP) repeats and six halftetratricopeptide (HAT) repeats at the C-terminal region. It is involved in the processing of a variety of RNAs and plays a critical role in the degradation of nuclear RNAs (PMID: 34514952).

Protocole

Product Specific Protocols
WB protocol for ZFC3H1 antibody 30834-1-APDownload protocol
IF protocol for ZFC3H1 antibody 30834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Fabienne (Verified Customer) (09-12-2025)

Works great in immunoprecipitation (used 3µg antibody per 250µg nuclear extracts)

  • Applications: Immunoprecipitation
  • Cell Tissue Type: Jurkat
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