Anticorps Recombinant de lapin anti-ZFP161 / ZBTB14

ZFP161 / ZBTB14 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240853B12

N° de cat : 83909-4-PBS

Synonymes

ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14

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Galerie de données de validation

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  WB analysis using 83909-4-RR (same clone as 83909-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83909-4-RR (ZFP161 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83909-4-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83909-4-RR (same clone as 83909-4-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZFP161 / ZBTB14 Recombinant antibody (83909-4-RR, Clone:240853B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83909-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83909-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83909-4-RR against Human ZFP161 / ZBTB14 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83909-4-PBS cible ZFP161 / ZBTB14 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZFP161 / ZBTB14 Protéine recombinante Ag16125
• Nom complet: zinc finger protein 161 homolog (mouse)
• Masse moléculaire calculée: 449 aa, 51 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC110519
• Symbole du gène: ZFP161
• Identification du gène (NCBI): 7541
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.

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