Anticorps Polyclonal de lapin anti-ZFP36

ZFP36 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(25)

N° de cat : 12737-1-AP

Synonymes

TTP, Zfp-36, Zfp 36, Tristetraprolin, mRNA decay activator protein ZFP36

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Galerie de données de validation

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  WB analysis using 12737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12737-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12737-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12737-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human bladder using 12737-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 12737-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 12737-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12737-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36 antibody (12737-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules A549, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu vésical humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• RIP: See 5 publications below

Informations sur le produit

12737-1-AP cible ZFP36 dans les applications de WB, IHC, IF/ICC, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZFP36 Protéine recombinante Ag3461
• Nom complet: zinc finger protein 36, C3H type, homolog (mouse)
• Masse moléculaire calculée: 326 aa, 34 kDa
• Poids moléculaire observé: 37-44 kDa
• Numéro d’acquisition GenBank: BC009693
• Symbole du gène: ZFP36
• Identification du gène (NCBI): 7538
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF). The calcualted molecular weight of ZFP36 is 34 kDa, but modified ZFP36 is about 40-45 kDa.

Publications

Species	Application	Title
human	WB,IF	Cell Res; Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses; Authors - Qihang Zhao
		Mol Cell; Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.; Authors - Ameya P Jalihal
mouse	WB,RIP	Dev Cell; N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.; Authors - Jie Du
		Proc Natl Acad Sci U S A; Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase.; Authors - Frederick E Tan
human,mouse	WB,IHC,IF	Acta Pharmacol Sin; Deletion of smooth muscle ZFP36 promotes neointimal hyperplasia in mice; Authors - Lei Wang ; KD Validated
human	WB,RIP	Am J Physiol Cell Physiol; Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1; Authors - Jie Zhao

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