Anticorps Polyclonal de lapin anti-ZFP36

ZFP36 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12737-1-PBS

Synonymes

TTP, Zfp-36, Zfp 36, Tristetraprolin, mRNA decay activator protein ZFP36

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Galerie de données de validation

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  WB analysis using 12737-1-AP (same clone as 12737-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 12737-1-AP (same clone as 12737-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 12737-1-AP (same clone as 12737-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12737-1-AP (same clone as 12737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IHC staining of human bladder using 12737-1-AP (same clone as 12737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12737-1-AP (same clone as 12737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IHC staining of human bladder using 12737-1-AP (same clone as 12737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12737-1-AP (same clone as 12737-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36 antibody (12737-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12737-1-PBS cible ZFP36 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZFP36 Protéine recombinante Ag3461
• Nom complet: zinc finger protein 36, C3H type, homolog (mouse)
• Masse moléculaire calculée: 326 aa, 34 kDa
• Poids moléculaire observé: 37-44 kDa
• Numéro d’acquisition GenBank: BC009693
• Symbole du gène: ZFP36
• Identification du gène (NCBI): 7538
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF). The calcualted molecular weight of ZFP36 is 34 kDa, but modified ZFP36 is about 40-45 kDa.

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