Anticorps Monoclonal anti-ZFP36

• Phare
• Validé par KD/KO

ZFP36 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1A5E6

N° de cat : 66938-1-Ig

Synonymes

G0S24, GOS24, NUP475, Protein TIS11A, RNF162A, TIS11, TIS11A, Tristetraproline, TTP, Zfp 36, ZFP36, Zinc finger protein 36 homolog

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Galerie de données de validation

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  WB analysis of HepG2 using 66938-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66938-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 66938-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66938-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 66938-1-Ig

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig lung using 66938-1-Ig

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66938-1-Ig (ZFP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules 3T3-L1, cellules A549, cellules NCI-H1299, cellules RAW 264.7, cellules U-937 traitées au LPS, tissu pulmonaire de porc

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66938-1-Ig cible ZFP36 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZFP36 Protéine recombinante Ag28473
• Nom complet: zinc finger protein 36, C3H type, homolog (mouse)
• Masse moléculaire calculée: 326 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC009693
• Symbole du gène: ZFP36
• Identification du gène (NCBI): 7538
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF).

Publications

Species	Application	Title
human	WB,IF,IP	Cell Rep; A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma; Authors - Yue Li
human	WB	Int Immunopharmacol; Angiotensin II contributes to intratumoral immunosuppressionvia induction of PD-L1 expression in non-small cell lung carcinoma.; Authors - Kaiyong Yang ; KD Validated