Anticorps Monoclonal anti-ZFP36

• Phare
• Validé par KD/KO

ZFP36 Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

WB

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5E6

N° de cat : CL488-66938

Synonymes

TTP, 1A5E6, Growth factor-inducible nuclear protein NUP475, mRNA decay activator protein ZFP36, Tristetraprolin

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Galerie de données de validation

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  WB analysis using CL488-66938

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66938 (ZFP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules NCI-H1299,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66938 cible ZFP36 dans les applications de WB et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZFP36 Protéine recombinante Ag28473
• Nom complet: zinc finger protein 36, C3H type, homolog (mouse)
• Masse moléculaire calculée: 326 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC009693
• Symbole du gène: ZFP36
• Identification du gène (NCBI): 7538
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF).

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