Anticorps Polyclonal de lapin anti-ZFP36L1/2

• Phare
• Validé par KD/KO

ZFP36L1/2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 12306-1-AP

Synonymes

Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1, ZFP36L1/2

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Galerie de données de validation

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  WB analysis of U-937 using 12306-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12306-1-AP

  WB result of ZFP36L1/2 antibody (12306-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZFP36L1/2 transfected HeLa cells.

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  WB analysis of HeLa using 12306-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 12306-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 12306-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 12306-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12306-1-AP (ZFP36L1/2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat ovary using 12306-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat ovary using 12306-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12306-1-AP

  Immunofluorescent analysis of HepG2 cells using 12306-1-AP (ZFP36L1/2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U-937, cellules 3T3-L1, cellules HeLa, cellules RAW 264.7
• Résultats positifs en IHC: tissu ovarien de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

12306-1-AP cible ZFP36L1/2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZFP36L1/2 Protéine recombinante Ag2952
• Nom complet: zinc finger protein 36, C3H type-like 1
• Masse moléculaire calculée: 338 aa, 36 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC018340
• Symbole du gène: ZFP36L1
• Identification du gène (NCBI): 677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZFP36L1, also named as Butyrate response factor 1 or TPA-induced sequence 11b, is a 338 amino acid protein, which is phosphorylated by RPS6KA1 at Ser-334 upon phorbol 12-myristate 13-acetate (PMA) treatment. ZFP36 encodes tristetraprolin (TTP) the prototype of a small family of RBPs, called the ZFP36 family, that are characterised by highly conserved tandem CCCH zinc-finger RNA-binding domains [PMID: 10751406]. ZFP36 is a RBP that promotes RNA decay and negatively regulates the expression of the myogenic regulatory factor MyoD by binding to the 3'UTR of MyoD mRNA [PMID: 25815583]. Mouse satellite cells from Zfp36-deficient mice express increased amounts of MyoD and display impaired satellite activation, demonstrating a role for ZFP36 in the maintenance of quiescence [PMID: 25815583]. The functions of the ZFP36L1 and ZFP36L2 family members have not been evaluated in skeletal muscle stem cell fate, but have been shown to act redundantly to promote quiescence during lymphocyte development. ZFP36L1 has been implicated in the persistence of the marginal zone B lymphocyte population. ZFP36L1 exists three isoform through blasting on NCBI database and can be phosphorylated, so the range of the molecular weight of ZFP36L1 is about 40-50 kDa. (PMID: 26180518, PMID: 17030620). This antibody recognizes both ZFP36L1 and ZFP36L2.

Publications

Species	Application	Title
human	IF	Cell; A Membraneless Organelle Associated with the Endoplasmic Reticulum Enables 3'UTR-Mediated Protein-Protein Interactions.; Authors - Weirui Ma
human	IF	Mol Ther Nucleic Acids; The miR-93-3p/ZFP36L1/ZFX axis regulates keratinocyte proliferation and migration during skin wound healing.; Authors - Xiao Feng ; KD Validated
mouse	WB,IHC,IF	Cell Death Dis; ZFP36L2 regulates myocardial ischemia/reperfusion injury and attenuates mitochondrial fusion and fission by LncRNA PVT1.; Authors - Fang Wu
human	WB	J Clin Invest; EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer; Authors - Xiaochao Tan ; KD Validated
human	WB	Elife; HUWE1 controls tristetraprolin proteasomal degradation by regulating its phosphorylation; Authors - Sara Scinicariello