Anticorps Polyclonal de lapin anti-ZFP64

ZFP64 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 17187-1-AP

Synonymes

Zfp 64, ZFP64, Zinc finger protein 338, ZNF338

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Galerie de données de validation

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  WB analysis of mouse spleen using 17187-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17187-1-AP (ZFP64 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17187-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17187-1-AP (ZFP64 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• ChIP: See 3 publications below

Informations sur le produit

17187-1-AP cible ZFP64 dans les applications de WB, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZFP64 Protéine recombinante Ag10971
• Nom complet: zinc finger protein 64 homolog (mouse)
• Masse moléculaire calculée: 681 aa, 75 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC041622
• Symbole du gène: ZFP64
• Identification du gène (NCBI): 55734
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZFP64 (also known as ZNF338) is a member of the Krüppel C2H2-type zinc finger family. ZFP64 is a coactivator of Notch1, mediating mesenchymal cell differentiation. ZFP64 expression was up-regulated in mouse peritoneal macrophages in response to stimulation with the TLR4 ligand LPS with a 2-fold increase. The MLL gene encoding the MLL fusion protein is easily activated by ZFP64. ZFP64 is essential for the continuous production of MLL fusion proteins in blood cancer and produces more cancer cells. Since ZFP64 deletion completely and specifically inhibits MLL fusion protein production and leukemia cascade growth, it is an ideal drug target.

Publications

Species	Application	Title
human	chIP	Cancer Cell; A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence.; Authors - Bin Lu
mouse	IF	Nat Neurosci; Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding; Authors - Ezgi Hacisuleyman
human	WB,ChIP	Biol Direct; ZFP64 drives glycolysis-mediated stem cell-like properties and tumorigenesis in breast cancer; Authors - Jiayi Sun
human	ChIP	J Transl Med; Cancer-associated fibroblasts promote doxorubicin resistance in triple-negative breast cancer through enhancing ZFP64 histone lactylation to regulate ferroptosis; Authors - KeJing Zhang

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