Anticorps Polyclonal de lapin anti-ZFX

ZFX Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 27655-1-AP

Synonymes

ZFX, zinc finger protein, X linked

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Galerie de données de validation

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  WB analysis using 27655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27655-1-AP (ZFX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules K-562, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27655-1-AP cible ZFX dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZFX Protéine recombinante Ag26576
• Nom complet: zinc finger protein, X-linked
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: ~130 kDa
• Numéro d’acquisition GenBank: NM_001178084
• Symbole du gène: ZFX
• Identification du gène (NCBI): 7543
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZFX (Zinc finger protein, X-linked), a key factor that controls the self-renewal of ESCs, belongs to the ZFY family whose members (ZFX, ZFY, ZFA) have similar molecular structures. Mammalian ZFX contains an acidic transcriptional activation domain, a nuclear localization signal (NLS) sequence, and a DNA binding domain consisting of 13 C2H2 zinc fingers. Knocking out ZFX has been shown to impair the self-renewal capacity of mouse ESC and tissue-specific adult stem cell such as hematopoietic stem cells (HSCs) without affecting their differentiation, and ZFX overexpression has been shown to promote ESC self-renewal by impeding differentiation. It's reported that t the 130 kDa band was N-glycosylated, and such a posttranslational modification (PTM) may control the nuclear import of ZFX, 91 kDa may be the non-N-glycosylated subtype which was lowly expressed. (PMID: 23322077, PMID:24228108)

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