Anticorps Polyclonal de lapin anti-ZFYVE16

• Phare
• Validé par KD/KO

ZFYVE16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13118-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 13118-2-AP (same clone as 13118-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 13118-2-AP (same clone as 13118-2-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 13118-2-AP (same clone as 13118-2-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 13118-2-AP (same clone as 13118-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

  IP result of anti-ZFYVE16 (IP:13118-2-AP, 5ug; Detection:13118-2-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE16 antibody (13118-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). CL594-Phalloidin (red). DAPI (blue) This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

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Informations sur le produit

13118-2-PBS cible ZFYVE16 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZFYVE16 Protéine recombinante Ag3811
• Nom complet: zinc finger, FYVE domain containing 16
• Masse moléculaire calculée: 1539 aa, 169 kDa
• Poids moléculaire observé: 230-250 kDa
• Numéro d’acquisition GenBank: BC032227
• Symbole du gène: ZFYVE16
• Identification du gène (NCBI): 9765
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)

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