Anticorps Polyclonal de lapin anti-SARA

• Phare
• Validé par KD/KO

SARA Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF

Conjugaison

Non conjugué

Publications

4

N° de cat : 14821-1-AP

Synonymes

hSARA, Madh interacting protein, MADHIP, Novel serine protease, NSP, Receptor activation anchor, SARA, SMADIP, ZFYVE9

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Galerie de données de validation

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  WB analysis of mouse brain using 14821-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14821-1-AP (SARA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

14821-1-AP cible SARA dans les applications de WB, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SARA Protéine recombinante Ag6592
• Nom complet: zinc finger, FYVE domain containing 9
• Masse moléculaire calculée: 1425 aa, 156 kDa
• Numéro d’acquisition GenBank: BC032680
• Symbole du gène: ZFYVE9
• Identification du gène (NCBI): 9372
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZFYVE9, also known as SARA (Smad anchor for receptor activation), is a double zinc finger (FYVE domain) protein that interacts directly with SMAD2 and SMAD3, and is involved in Alzheimer's disease. SARA functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. SARA plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. SARA is possibly associated with TGF-beta receptor internalization.

Publications

Species	Application	Title
mouse	IF	Mol Psychiatry; WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling.; Authors - Min Wang ; KD Validated
human	WB	Oncotarget; TGFBR-IDH1-Cav1 axis promotes TGF-β signalling in cancer-associated fibroblast.; Authors - Xiaodan Hou
human	WB, IF	J Biol Chem; Phosphatidylinositol 3-kinase and Rab5 GTPase inversely regulate the Smad anchor for receptor activation (SARA) protein independently of transforming growth factor-β1.; Authors - Runyan Constance E CE
mouse	WB	Stem Cell Reports; Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool.; Authors - Xuanmao Jiao