Anticorps Monoclonal anti-ZG16

ZG16 Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A7B9

N° de cat : 67389-1-PBS

Synonymes

1A7B9, FLJ43571, FLJ92276, hZG16, JCLN

---

Galerie de données de validation

• 

  WB analysis of mouse colon using 67389-1-Ig (same clone as 67389-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 67389-1-Ig (same clone as 67389-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 67389-1-Ig (same clone as 67389-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CD163 antibody (83285-4-RR, Clone: 240233A3, red). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67389-1-PBS cible ZG16 dans les applications de WB, IHC, IF-P, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZG16 Protéine recombinante Ag11332
• Nom complet: zymogen granule protein 16 homolog (rat)
• Masse moléculaire calculée: 167 aa, 18 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC029149
• Symbole du gène: ZG16
• Identification du gène (NCBI): 653808
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)

{{ptg:RelatedPrimaryAntibodies}}