Anticorps Polyclonal de lapin anti-ZHX1

• Phare
• Validé par KD/KO

ZHX1 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13903-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 13903-1-AP (same clone as 13903-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 13903-1-AP (same clone as 13903-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 13903-1-AP (same clone as 13903-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 13903-1-AP (same clone as 13903-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 13903-1-AP (same clone as 13903-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13903-1-AP (same clone as 13903-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13903-1-AP (same clone as 13903-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 13903-1-AP (same clone as 13903-1-PBS)

  IP result of anti-ZHX1 (IP:13903-1-AP, 4ug; Detection:13903-1-AP 1:500) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13903-1-PBS cible ZHX1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZHX1 Protéine recombinante Ag4874
• Nom complet: zinc fingers and homeoboxes 1
• Masse moléculaire calculée: 873 aa, 98 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC040481
• Symbole du gène: ZHX1
• Identification du gène (NCBI): 11244
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Binding of the nuclear factor-Y complex (NF-Y) to the inverted CCAAT-box interferes with transcription activation through nucleosome reorganization. The three homologous proteins forming the zinc-fingers and homeoboxes (ZHX) family interact with the activation domain of NF-Ya to repress transcription [PMID:19348505,20509910]. Human zinc-fingers and homeoboxes 1 (ZHX1) was cloned as a protein that interacts with the activation domain (AD) of the A subunit of nuclear factor-Y (NF-YA) [PMID:12237128].

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