Anticorps Monoclonal anti-ZHX2

• Phare
• Validé par KD/KO

ZHX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4E12F2

N° de cat : 68268-1-PBS

Synonymes

RAF, KIAA0854, Alpha-fetoprotein regulator 1, AFR1, AFP regulator 1

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Galerie de données de validation

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  WB analysis using 68268-1-Ig (same clone as 68268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68268-1-Ig (ZHX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68268-1-Ig (same clone as 68268-1-PBS)

  WB result of ZHX2 antibody (68268-1-Ig; 1:24000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZHX2 transfected HEK-293 cells. This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68268-1-Ig (same clone as 68268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68268-1-Ig (ZHX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68268-1-Ig (same clone as 68268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68268-1-Ig (ZHX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68268-1-Ig (same clone as 68268-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZHX2 antibody (68268-1-Ig, Clone: 4E12F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68268-1-PBS cible ZHX2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZHX2 Protéine recombinante Ag14420
• Nom complet: zinc fingers and homeoboxes 2
• Masse moléculaire calculée: 837 aa, 92 kDa
• Poids moléculaire observé: 92-100 kDa
• Numéro d’acquisition GenBank: BC042145
• Symbole du gène: ZHX2
• Identification du gène (NCBI): 22882
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

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