Anticorps Polyclonal de lapin anti-ZIC3

ZIC3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 21717-1-AP

Synonymes

ZNF203, Zinc finger protein ZIC 3

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Galerie de données de validation

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  WB analysis of Neuro-2a using 21717-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 21717-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 21717-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 21717-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 21717-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 21717-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 21717-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules SH-SY5Y, cellules Y79, tissu de cervelet de souris
• Résultats positifs en IHC: tissu de carcinome urothélial humain, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer de la thyroïde humain, tissu de cervelet de rat, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21717-1-AP cible ZIC3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZIC3 Protéine recombinante Ag16354
• Nom complet: Zic family member 3 (odd-paired homolog, Drosophila)
• Masse moléculaire calculée: 467 aa, 51 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC113395
• Symbole du gène: ZIC3
• Identification du gène (NCBI): 7547
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

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