Anticorps Polyclonal de lapin anti-ZIP8

• Phare
• Validé par KD/KO

ZIP8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20459-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse lung using 20459-1-AP (same clone as 20459-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 20459-1-AP (same clone as 20459-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 20459-1-AP (same clone as 20459-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 20459-1-AP (same clone as 20459-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 20459-1-AP (same clone as 20459-1-PBS)

  IP result of anti-ZIP8 (IP:20459-1-AP, 4ug; Detection:20459-1-AP 1:500) with mouse liver tissue lysate 6000ug. This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 20459-1-AP (same clone as 20459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 20459-1-AP (ZIP8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20459-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20459-1-PBS cible ZIP8 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZIP8 Protéine recombinante Ag14292
• Nom complet: solute carrier family 39 (zinc transporter), member 8
• Masse moléculaire calculée: 460 aa, 50 kDa
• Poids moléculaire observé: 42-46 kDa, 75-90 kDa
• Numéro d’acquisition GenBank: BC012125
• Symbole du gène: ZIP8
• Identification du gène (NCBI): 64116
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC39A8, also known as ZIP8, belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Recently it was found that ZIP8 expression is upregulated in human monocytes in response to LPS, TNF-α, and live bacteria, facilitating cytoprotection during the early inflammation. Besides zinc ZIP8 can also transport cadmium and manganese efficiently. It is predicted that ZIP8 contains 3 potential N-linked glycosylation sites and is subject to glycosylation, which may account for the presences of multiple molecular weights, such as 43 kDa, 49 kDa, 60 kDa, 75-90 kDa, 150 kDa, and 200 kDa.

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