Anticorps Polyclonal de lapin anti-ZMYND10

• Phare
• Validé par KD/KO

ZMYND10 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 14431-1-AP

Synonymes

BLU, LUCA12.4, Protein BLu, Zinc finger MYND domain-containing protein 10

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Galerie de données de validation

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  WB analysis using 14431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14431-1-AP (ZMYND10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14431-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14431-1-AP (ZMYND10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 14431-1-AP

  IP result of anti-ZMYND10 (IP:14431-1-AP, 4ug; Detection:14431-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules SH-SY5Y, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

14431-1-AP cible ZMYND10 dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZMYND10 Protéine recombinante Ag5808
• Nom complet: zinc finger, MYND-type containing 10
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC033732
• Symbole du gène: ZMYND10
• Identification du gène (NCBI): 51364
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZMYND10 (Zinc finger MYND domain-containing protein 10), also known as BLU, encodes a 50-kDa protein containing an MYND-type zinc finger DNA-binding domain in the C-terminus that is commonly found in transcription repressors. ZMYND10 is highly enriched in ciliated cells compared with that in nonciliated cells and is expressed in motile ciliated tissues in mice (PMID: 23891471). ZMYND10 is a tumor suppressor that can induce apoptosis, arrest cell cycle, and inhibit proliferation and angiogenesis in different tumors (PMID: 29601588, 31801619).

Publications

Species	Application	Title
mouse	WB,IP,IF	Elife; ZMYND10 functions in a chaperone relay during axonemal dynein assembly.; Authors - Girish R Mali ; KO Validated
human	WB,IF	Andrology; A novel homozygous LRRC6 mutation causes male infertility with asthenozoospermia and primary ciliary dyskinesia in humans; Authors - Shengjia Shi
rat	WB	Environ Toxicol; Sodium arsenite impairs sperm quality via downregulating the ZMYND15 and ZMYND10; Authors - Xiangli Li

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