Anticorps Recombinant de lapin anti-ZNF148

ZNF148 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241632D8

N° de cat : 84338-7-RR

Synonymes

Transcription factor ZBP 89, pHZ 52, HT BETA, BFCOL1, BERF 1

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Galerie de données de validation

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  WB analysis using 84338-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84338-7-RR (ZNF148 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84338-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84338-7-RR against Human ZNF148 were performed. The affinity constant is 1.85 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HCT 116, cellules HeLa, cellules HepG2, cellules MDA-MB-231, cellules NIH/3T3, cellules T-47D

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84338-7-RR cible ZNF148 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZNF148 Protéine recombinante Ag35742
• Nom complet: zinc finger protein 148
• Poids moléculaire observé: 103 kDa
• Numéro d’acquisition GenBank: BC035591
• Symbole du gène: ZNF148
• Identification du gène (NCBI): 7707
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Kruppel-like zinc finger protein 148 (ZNF148), also known as β enolase repressor factor 1 (BERF1, ZBP-89, and BFCOL1), encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. ZNF148 participates in the regulation of cell proliferation and death, and is expressed at lower levels in mammalian somatic cells, and highly expressed in tumor cells. Previous studies show that there was a significant correlation between ZNF148 expression and the invasion and metastasis of colorectal tumors. ZNF148 has two alternative splicing isoforms, which are ZNF148FL and ZNF148ΔN. ZNF148FL contains a complete 794 amino acids, ZNF148ΔN lacks the amino-terminal 129 amino acids, and the mechanisms involved in the generation of these two splicing isoforms are not yet clear (PMID: 30463804). Thus, ZNF148 can be observed to have two molecular weights near 103 kDa.

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