Anticorps Recombinant de lapin anti-ZNF217

• Phare
• Validé par KD/KO

ZNF217 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241217E3

N° de cat : 84276-1-RR

Synonymes

ZFP217, 241217E3, ZABC1, Zinc finger protein 217

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Galerie de données de validation

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  WB analysis using 84276-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84276-1-RR (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 84276-1-RR

  WB result of ZNF217 antibody (84276-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF217 transfected HEK-293 cells.

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  IF Staining of HepG2 using 84276-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 84276-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNF217 antibody (84276-1-RR, Clone: 241217E3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84276-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84276-1-RR against Human ZNF217 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Caco-2, cellules HEK-293, cellules LNCaP, cellules PC-3
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84276-1-RR cible ZNF217 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZNF217 Protéine recombinante Ag19453
• Nom complet: zinc finger protein 217
• Masse moléculaire calculée: 1048 aa, 115 kDa
• Poids moléculaire observé: ~ 130 kDa
• Numéro d’acquisition GenBank: BC113427
• Symbole du gène: ZNF217
• Identification du gène (NCBI): 7764
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zinc finger protein 217 (ZNF217) is a member of the Krüppel-like factor transcription factor family. ZNF217 possesses a characteristic structure of zinc finger motifs and plays a crucial role in regulating the biological activities of cells. ZNF217 binds to DNA through its zinc fingers thereby targeting potential protein binding partners to specific sites in the genome. Recent findings have revealed that ZNF217 is strongly associated with multiple aspects of cancer progression, impacting patient prognosis. PMID: 38259608

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