Anticorps Recombinant de lapin anti-ZNF350

ZNF350 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241225D5

N° de cat : 84121-5-RR

Synonymes

Zinc finger protein ZBRK1, zinc finger protein 350, ZFQR, ZBRK1, KRAB zinc finger protein ZFQR

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Galerie de données de validation

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  WB analysis of THP-1 using 84121-5-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84121-5-RR (ZNF350 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 84121-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug ZNF350 Recombinant antibody (84121-5-RR, Clone:241225D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84121-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84121-5-RR against Human ZNF350 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules THP-1,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84121-5-RR cible ZNF350 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZNF350 Protéine recombinante Ag23982
• Nom complet: zinc finger protein 350
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC009921
• Symbole du gène: ZNF350
• Identification du gène (NCBI): 59348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZNF350 (Zinc finger protein 350), also known as zinc-finger and BRCA1-interacting protein with a Kruppel-associated box (KRAB) domain (ZBRK1), is involved in the development of several human tumor types, including breast, colon, and cervical carcinomas. ZNF350 has been identified as a transcriptional suppressor that can either form complexes with other proteins or play a direct transcriptional suppressive role in a single-factor form (PMID: 30613364). Overexpression of ZBRK1 has the potential to inhibit cancer cell migration and suppress metastasis activity suggesting that ZBRK1 may behave as a metastasis suppressor (PMID: 19996286).

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